Anti Lamin A Antibody

Since both dna blue and lamin a c red are associated with the nuclear compartment this region appears crimson in this image.

Anti lamin a antibody.

Anti lamin a c antibodies are available from several suppliers. The protein may also be known as lamin c dhe lamin a cdcd1 cddc and cmd1a. 74 kda observed band size. See machiels et al 1997.

The anti lamin a c antibody reveals strong nuclear lamina staining while anti lamp1 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes. In humans this protein is encoded by the gene lmna. This antibody can also be used in indirect immunofluorescence 1 2 μg ml using hela cells rat nrk and mouse 3t3 cells. 2000 j struct biol 129 313 23.

The full protein is reported to be 664 amino acid residues in length. Anti lamin a c terminal antibody is suitable for use in western blot 0 1 0 2 μg ml using hela nuclear extract. It is listed to be a member of the intermediate filament family. Reacts against lamin a exclusively the antibody was raised against the carboxy terminus of 98 amino acids present in lamin a and absent from lamin c.

The cleavage of lamins results in nuclear dysregulation and cell death 5 6. Lamin a c antibodies. Lamin a antibody c 3 is a mouse monoclonal igg 2b kappa light chain provided at 200 µg ml. Why is the actual band size different from the predicted.

The cells were labeled with lamin a monoclonal antibody 133a2 product ma1 06101 at 5 µg ml in 0 1 bsa and incubated overnight at 4 degree and then labeled with goat anti mouse igg h l superclonal secondary antibody alexa fluor 488 conjugate product a28175 at a dilution of 1 2000 for 45 minutes at room temperature panel a. Additionally anti lamin a c terminal antibodies are suitable for use in immunoblotting approx. Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms a b and c.

The expected protein mass is 74 1 kda but there are 6 reported isoforms. Specific for an epitope mapping between amino acids 580 601 near the c terminus of lamin a of human origin. As a result this antibody recognizes lamin a but not lamin c. Lamins a and c are alternatively spliced versions of the lmna gene.

Anti lamin a antibody c 3 is recommended for detection of lamin a of mouse rat and human origin by wb ip if and elisa. Anti lamin a antibodies are readily available from several suppliers. During apoptosis lamin a c is specifically cleaved into a large 41 50 kda and a small 28 kda fragment 3 4.