Anti Lamin Antibody What Is It

Expression of drosophila lamin c is developmentally regulated.

Anti lamin antibody what is it.

Lamins a and c are alternatively spliced versions of the lmna gene. Anti lamin a c terminal antibody is suitable for use in western blot 0 1 0 2 μg ml using hela nuclear extract. Weber k journal of cell science 108 pt 10. Specific for an epitope mapping between amino acids 580 601 near the c terminus of lamin a of human origin.

The full protein is reported to be 664 amino acid residues in length. However based on bioassays it appears that the mix of antibodies in this purified polyclonal anti serum bind multiply across the large laminin molecule to an extent that its biological activities are inhibited. Performed under reducing conditions. Analogies with vertebrate a type lamins.

Lamin a antibody c 3 is a mouse monoclonal igg 2b kappa light chain provided at 200 µg ml. Anti lamin a antibody ab26300 at 1 µg ml lane 1. Goat anti rabbit igg h l hrp preadsorbed at 1 5000 dilution developed using the ecl technique. Anti lamin antibodies are available from several suppliers.

It is the best among many laminin function blocking preps we have tested. Fisher pa journal of cell science 108 pt 9. These proteins are found in many different cell types in three different forms a b and c. Species predicted to react based on 100 sequence homology.

Site mapping using a monoclonal antibody. Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. That is cell receptor binding is markedly hindered. Additionally anti lamin a c terminal antibodies are suitable for use in immunoblotting approx.

Anti lamin a c antibody e 1 is a mouse monoclonal igg 1 kappa light chain lamin a c antibody provided at 200 µg ml specific for an epitope mapping between amino acids 2 29 at the n terminus of lamin a c of human origin. Lamin a c antibody detects endogenous levels of total full length lamin a and lamin c 70 kda as well as the small fragment of lamin a and lamin c resulting from cleavage at aspartic acid 230 28 kda. Anti lamin antibody products lamin is a reported alias of the human protein lamin a c encoded by the gene lmna. Anti lamin a antibody c 3 is recommended for detection of lamin a of mouse rat and human origin by wb ip if and elisa.

This antibody can also be used in indirect immunofluorescence 1 2 μg ml using hela cells rat nrk and mouse 3t3 cells. Lamin a c antibodies lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. Recombinant human lamin a protein at 0 01 µg secondary all lanes.